Production methods and properties of virus-like particles of Saccharomyces cerevisiae yeast killer strain

被引：0
|
作者：
Z. A. Shevchenko
L. R. Lebedev
V. P. Klimenko
V. I. Masycheva
S. G. Gamaley
A. V. Bateneva
E. D. Danilenko
E. A. Ilyasov
M. Sh. Azaev
B. N. Zaytsev
机构：
[1] State Research Center of Virology and Biotechnology VECTOR,
来源：
Applied Biochemistry and Microbiology | 2015年 / 51卷
关键词：
virus-like particle; double-stranded RNA; interferon induction; capsid protein; RNase; phagocytosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Based on the technology for producting virus-like particles of a killer strain of Saccharomyces cerevisiae Y-448 yeast, which includes stages of extraction, concentration, and chromatographic purification, experimental preparation samples were obtained and their properties were researched. The particle size was detected by electron microscopy. It was identified that dsRNA, which is in the composition of particles, is notable for its higher enzymatic stability as compared to loose dsRNA. It was shown in in vivo experiments that the obtained VLP preparations increase the phagocytic activity of macrophages and the interferon level in mouse blood serum to a greater extent than the drug of comparison, Rhidostin.
引用
收藏
页码：812 / 817
页数：5
相关论文
共 50 条
  • [31] Trans-packaging of human immunodeficiency virus type 1 genome into Gag virus-like particles in Saccharomyces cerevisiae
    Tomo, Naoki
    Goto, Toshiyuki
    Morikawa, Yuko
    MICROBIAL CELL FACTORIES, 2013, 12
  • [32] Generation of recombinant porcine parvovirus virus-like particles in Saccharomyces cerevisiae and development of virus-specific monoclonal antibodies
    Burneikiene, Rasa Petraityte
    Tamosiunas, Paulius Lukas
    Akatov, Artiomas
    Lasickiene, Rita
    Kundrotas, Gabrielis
    Sereika, Vilimas
    Lelesius, Raimundas
    Zvirbliene, Aurelija
    Sasnauskas, Kestutis
    JOURNAL OF BIOTECHNOLOGY, 2014, 185 : S39 - S39
  • [33] Trans-packaging of human immunodeficiency virus type 1 genome into Gag virus-like particles in Saccharomyces cerevisiae
    Naoki Tomo
    Toshiyuki Goto
    Yuko Morikawa
    Microbial Cell Factories, 12
  • [34] Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies
    Tamosiunas, Paulius Lukas
    Petraityte-Burneikiene, Rasa
    Lasickiene, Rita
    Akatov, Artiomas
    Kundrotas, Gabrielis
    Sereika, Vilimas
    Lelesius, Raimundas
    Zvirbliene, Aurelija
    Sasnauskas, Kestutis
    JOURNAL OF IMMUNOLOGY RESEARCH, 2014, 2014
  • [35] Production of La Crosse virus-like particles
    Hughes, Mark T.
    Blair, Carol D.
    Beaty, Barry J.
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2006, 75 (05): : 185 - 186
  • [36] Virus-Like Particles as an Instrument of Vaccine Production
    Syomin, B. V.
    Ilyin, Y. V.
    MOLECULAR BIOLOGY, 2019, 53 (03) : 323 - 334
  • [37] Virus-Like Particles as an Instrument of Vaccine Production
    B. V. Syomin
    Y. V. Ilyin
    Molecular Biology, 2019, 53 : 323 - 334
  • [38] Virus-like particles production in green plants
    Santi, Luca
    Huang, Zhong
    Mason, Hugh
    METHODS, 2006, 40 (01) : 66 - 76
  • [39] VIRUS-LIKE PARTICLES IN ABNORMAL CELLS OF SACCHAROMYCES-CARLSBERGENSIS
    VOLKOFF, O
    WALTERS, T
    CANADIAN JOURNAL OF GENETICS AND CYTOLOGY, 1970, 12 (03): : 621 - &
  • [40] INDUCTION OF TY1 VIRUS-LIKE PARTICLES BY MINI-TY1 IN SPT3 STRAIN OF SACCHAROMYCES-CEREVISIAE
    REZNIK, NL
    ZOLOTOVA, LI
    SCHUPPE, NG
    DOKLADY AKADEMII NAUK, 1995, 340 (01) : 138 - 140
12345