Production methods and properties of virus-like particles of Saccharomyces cerevisiae yeast killer strain

被引：0
|
作者：
Z. A. Shevchenko
L. R. Lebedev
V. P. Klimenko
V. I. Masycheva
S. G. Gamaley
A. V. Bateneva
E. D. Danilenko
E. A. Ilyasov
M. Sh. Azaev
B. N. Zaytsev
机构：
[1] State Research Center of Virology and Biotechnology VECTOR,
来源：
Applied Biochemistry and Microbiology | 2015年 / 51卷
关键词：
virus-like particle; double-stranded RNA; interferon induction; capsid protein; RNase; phagocytosis;
D O I：
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摘要：
Based on the technology for producting virus-like particles of a killer strain of Saccharomyces cerevisiae Y-448 yeast, which includes stages of extraction, concentration, and chromatographic purification, experimental preparation samples were obtained and their properties were researched. The particle size was detected by electron microscopy. It was identified that dsRNA, which is in the composition of particles, is notable for its higher enzymatic stability as compared to loose dsRNA. It was shown in in vivo experiments that the obtained VLP preparations increase the phagocytic activity of macrophages and the interferon level in mouse blood serum to a greater extent than the drug of comparison, Rhidostin.
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页码：812 / 817
页数：5
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