Novel ratiometric fluorescent probe based on internal reference and its detection of hydrazine

In this work, dual-emissive ratiometric fluorescent system was constructed by the introduction of an ideal internal reference. By virtue of its unique alkalinity, N2H4 could undergo a hydrazinolysis reaction with the ester group of F1, inducing remarkable fluorescence enhancement while the blue fluorescence of the internal reference DPA remained constant. Consequently, the fluorescence intensity ratios (I540/I440) were proportional to the concentrations of N2H4, which was beneficial for the exactly quantitative detection. The skillful strategy granted the sensing system advantages such as relative good solubility in aqueous media, easy-to-design, simple synthesis, large emission shift, good ratiometric response, as well as the successful application in real water samples and cell imaging.