Detection of a Double-Stranded RNA Virus from a Strain of the Violet Root Rot Fungus Helicobasidium mompa Tanaka

被引:0
|
作者
Hideki Osaki
Kinya Nomura
Toru Iwanami
Satoko Kanematsu
Ikuko Okabe
Naoyuki Matsumoto
Atsuko Sasaki
Yoshihiro Ohtsu
机构
[1] National Institute of Fruit Tree Science,Apple Research Center
[2] National Institute of Fruit Tree Science,undefined
来源
Virus Genes | 2002年 / 25卷
关键词
dsRNA; partitivirus; RNA-dependent RNA polymerase;
D O I
暂无
中图分类号
学科分类号
摘要
Three double-stranded (ds) RNA species (ca. 1.30, 1.27 and 1.23×106) were isolated by CF-11 cellulose chromatography from a strain of the violet root rot fungus Helicobasidium mompa recovered from apple roots. Purified virion preparations contained isometric particles about 25nm in diameter, and also the same three species of dsRNA isolated from total extracts by CF-11 cellulose chromatography. The molecular mass of the coat protein was about 67K when estimated by SDS-PAGE. The largest dsRNA (referred to as dsRNA1) contains a single, long open reading frame of 1794 nucleotides that encodes a putative polypeptide containing 598 amino acid residues with a molecular mass of 69.9K. This polypeptide contains amino acid sequence motifs conserved in putative RNA-dependent RNA polymerases of RNA viruses. Phylogenetic analysis revealed similarities to RNA-dependent RNA polymerases from Atkinsonella hypoxylon 2H virus, a member of the family Partitiviridae.
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页码:139 / 145
页数:6
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