Purification and Properties of Poly(3-Hydroxybutyrate) Depolymerase from the Fungus Paecilomyces lilacinus D218

被引：0

作者：

Yuji Oda

Hidekazu Osaka

Teizi Urakami

Kenzo Tonomura

机构：

[1] Department of Food Science and Technology,

[2] Fukuyama University,undefined

[3] Fukuyama,undefined

[4] Hiroshima 729-02,undefined

[5] Japan ,undefined

[6] Mitsubishi Gas Chemical Company,undefined

[7] Chiyoda-ku,undefined

[8] Tokyo 100,undefined

[9] Japan ,undefined

来源：

Current Microbiology | 1997年 / 34 卷

关键词：

Enzyme; Molecular Weight; Chromatography; Purification; Triglyceride;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Poly(3-hydroxybutyrate) depolymerase was purified to homogeneity from the culture filtrate of Paecilomyces lilacinus D218 by column chromatography on CM-Toyopearl 650M and hydroxylapatite. The molecular weight of the enzyme was estimated to be 48,000 by SDS-PAGE. Maximal activity was observed near pH 7.0 and 45°C. The Km and Vmax values for PHB were 0.13 (mg/ml) and 3750 (U/mg protein), respectively. The enzyme hydrolyzed PHB and p-nitrophenyl fatty acids but not polycaprolactone and triglycerides.

引用

页码：230 / 232

页数：2

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