Purification and Properties of Poly(3-Hydroxybutyrate) Depolymerase from the Fungus Paecilomyces lilacinus D218

被引：0
|
作者：
Yuji Oda
Hidekazu Osaka
Teizi Urakami
Kenzo Tonomura
机构：
[1] Department of Food Science and Technology,
[2] Fukuyama University,undefined
[3] Fukuyama,undefined
[4] Hiroshima 729-02,undefined
[5] Japan ,undefined
[6] Mitsubishi Gas Chemical Company,undefined
[7] Chiyoda-ku,undefined
[8] Tokyo 100,undefined
[9] Japan ,undefined
来源：
Current Microbiology | 1997年 / 34 卷
关键词：
Enzyme; Molecular Weight; Chromatography; Purification; Triglyceride;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Poly(3-hydroxybutyrate) depolymerase was purified to homogeneity from the culture filtrate of Paecilomyces lilacinus D218 by column chromatography on CM-Toyopearl 650M and hydroxylapatite. The molecular weight of the enzyme was estimated to be 48,000 by SDS-PAGE. Maximal activity was observed near pH 7.0 and 45°C. The Km and Vmax values for PHB were 0.13 (mg/ml) and 3750 (U/mg protein), respectively. The enzyme hydrolyzed PHB and p-nitrophenyl fatty acids but not polycaprolactone and triglycerides.
引用
收藏
页码：230 / 232
页数：2
相关论文
共 50 条
  • [1] Purification and properties of poly(3-hydroxybutyrate) depolymerase from the fungus Paecilomyces lilacinus D218
    Oda, Y
    Osaka, H
    Urakami, T
    Tonomura, K
    CURRENT MICROBIOLOGY, 1997, 34 (04) : 230 - 232
  • [2] Purification and Characterization of Fungal Poly(3-hydroxybutyrate) Depolymerase from Paecilomyces lilacinus F4-5 and Enzymatic Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Film
    Byoung-In Sang
    Won-Kwon Lee
    Katsutoshi Hori
    Hajime Unno
    World Journal of Microbiology and Biotechnology, 2006, 22 : 51 - 57
  • [3] Purification and characterization of fungal poly(3-hydroxybutyrate) depolymerase from Paecilomyces lilacinus F4-5 and enzymatic degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) film
    Sang, BI
    Lee, WK
    Hori, K
    Unno, H
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2006, 22 (01): : 51 - 57
  • [4] Properties of a Poly(3-hydroxybutyrate) Depolymerase from Penicillium funiculosum
    Sakie Miyazaki
    Kazuhei Takahashi
    Mari Shiraki
    Terumi Saito
    Yoko Tezuka
    Ken-ichi Kasuya
    Journal of Polymers and the Environment, 2000, 8 : 175 - 182
  • [5] Properties of a poly(3-hydroxybutyrate) depolymerase from Penicillium funiculosum
    Miyazaki, S
    Takahashi, K
    Shiraki, M
    Saito, T
    Tezuka, Y
    Kasuya, K
    JOURNAL OF POLYMERS AND THE ENVIRONMENT, 2000, 8 (04) : 175 - 182
  • [6] Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerase produced by Penicillium pinophilum
    Han, JS
    Son, YJ
    Chang, CS
    Kim, MN
    JOURNAL OF MICROBIOLOGY, 1998, 36 (02) : 67 - 73
  • [7] Purification and Properties of Extracellular Poly(3-hydroxybutyrate) Depolymerase Produced by Aspergillus fumigatus 202
    Rachana Bhatt
    K. C. Patel
    Ujjval Trivedi
    Journal of Polymers and the Environment, 2010, 18 : 141 - 147
  • [8] Purification and Properties of Extracellular Poly(3-hydroxybutyrate) Depolymerase Produced by Aspergillus fumigatus 202
    Bhatt, Rachana
    Patel, K. C.
    Trivedi, Ujjval
    JOURNAL OF POLYMERS AND THE ENVIRONMENT, 2010, 18 (02) : 141 - 147
  • [9] Molecular characterization of a poly(3-hydroxybutyrate) depolymerase from a filamentous fungus, penicillium funiculosum.
    Kasuya, K
    Tezuka, Y
    Ishii, N
    Yamagata, Y
    Hamai, Y
    Shiraki, M
    Saito, T
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 229 : U945 - U945
  • [10] DEGRADATION OF POLY(3-HYDROXYBUTYRATE) BY POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE FROM ALCALIGENES-FAECALIS T-1
    SHIRAKURA, Y
    FUKUI, T
    SAITO, T
    OKAMOTO, Y
    NARIKAWA, T
    KOIDE, K
    TOMITA, K
    TAKEMASA, T
    MASAMUNE, S
    BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 880 (01) : 46 - 53
12345