Site-directed mutagenesis of bovine FGF-2 cDNA allows the production of the human-form of FGF-2 in Escherichia coli

被引：0
|
作者：
M.L.S. Oliveira
J.A. Coutinho
J.E. Krieger
I. Raw
P.L. Ho
机构：
[1] Centro de Biotecnologia,Departamento de Bioquímica, Faculdade de Medicina de Ribeirão Preto
[2] Instituto Butantan,undefined
[3] Universidade de São Paulo,undefined
[4] Faculdade de Medicina da Universidade de São Paulo,undefined
[5] Instituto do Coração (InCor),undefined
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
angiogenesis; fibroblast growth factor 2; recombinant protein;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Human fibroblast growth factor-2 can be used to induce angiogenesis in ischemias and wound healing. Site-directed mutagenesis of bovine FGF-2 cDNA was performed in order to produce the human-form of FGF-2 in E. coli. The mitogenic, angiogenic and neurotrophic activities of the recovered protein were analysed by [3H]thymidine uptake to DNA of cultured fibroblasts, rabbit ear dermal ulcers wound healing and neuronal differentiation of PC12 cells.
引用
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页码：1151 / 1157
页数：6
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