Identification and genetic characterization of new bovine viral diarrhea virus genotype 2 strains in pigs isolated in China

被引:0
|
作者
Jie Tao
Yin Wang
Juan Wang
Jian-ye Wang
Guo-qiang Zhu
机构
[1] College of Veterinary Medicine,
[2] Yangzhou University,undefined
来源
Virus Genes | 2013年 / 46卷
关键词
BVDV-2; Pig; Noncytopathogenic; Complete genome;
D O I
暂无
中图分类号
学科分类号
摘要
Classical swine fever (CSF)-like symptoms in pigs regarded as free from CSF has been reported previously. From sick pigs with CSF-like symptoms, and who had been inoculated with the hog cholera vaccine, samples were collected and subjected to RT-PCR using specific primers. Twelve bovine viral diarrhea virus 2 (BVDV-2) strains were screened and isolated. Homology comparison showed that the E2 genes of the twelve isolates were highly conserved. The genome of the one of the BVDV-2 isolates (named as SH-28) from the sick pigs, which showed a noncytopathic effect in MDBK cell cultures and strong reactivity with monoclonal antibody (MAb) Bz-53 raised against BVDV-2, was sequenced. The genome of SH-28 comprises 12,279 nucleotides and contains a large open reading frame beginning at nucleotide 386 and ending at nucleotide 12,073. Genomic comparison and phylogenetic analyzes showed that SH-28 fall into BVDV-2 subtype and was most similar to XJ-04 (nucleotide and amino acid homologies were 89.9–93.8 % and 91.1–96.9 %, respectively), but was genetically divergent from ZM-95 (pig BVDV-1).
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页码:81 / 87
页数:6
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