Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana

被引：0
|
作者：
Xiaoguang Sheng
Fan Liu
Yuelin Zhu
Hong Zhao
Li Zhang
Bin Chen
机构：
[1] Nanjing Agricultural University,College of Horticulture
[2] Beijing Vegetable Research Center,undefined
[3] Beijing Academy of Agriculture and Forestry Sciences,undefined
来源：
Plant Cell, Tissue and Organ Culture | 2008年 / 92卷
关键词：
Intergeneric hybridization; Hybridity verification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The gene pool of Brassica oleracea was enriched via intergeneric somatic hybridization between B. oleracea (2n = 18) and Matthiola incana (2n = 14). One hundred and eighteen plants were obtained from 96 calli. Only four plants (H1, H2, H3 and H4) showed an intermediate phenotype from the parents; among these, H1 and H3 arose from the same callus. Random amplified polymorphic DNA (RAPD), sequence-related amplified polymorphism (SRAP), and cytological analyses confirmed that H1 and H3 were hybrids. The nuclear DNA content of the regenerated plants was determined by flow cytometry. More than half of the plants contained a nuclear DNA content of 1.3 to 3.9 pg/cell, which was higher than the content of B. oleracea but lower than that of M. incana. H1 contained 4.89 ± 0.02 pg of DNA per cell, while H3 nuclear DNA content was estimated at 4.87 ± 0.06 pg/cell. Cytological study of the root-tip cells revealed that the majority of the H1 and H3 hybrid cells contained 28 chromosomes.
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页码：55 / 62
页数：7
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