Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato

被引：0
|
作者：
A. S. Speranskaya
A. A. Krinitsina
P. Poltronieri
P. Fasano
A. Santino
A. B. Shevelev
T. A. Valueva
机构：
[1] Russian Academy of Sciences,Bach Institute of Biochemistry
[2] National Research Council of Italy,undefined
[3] ISPA-CNR,undefined
来源：
Biochemistry (Moscow) | 2005年 / 70卷
关键词：
Kunitz-type proteinase inhibitors; gene sequences; genome; potato;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Eighteen clones representing copies of four Kunitz-type proteinase inhibitor group B genes (PKPI-B) obtained by polymerase chain reaction cloning of potato (Solanum tuberosum L. cv. Istrinskii) genomic DNA were sequenced and analyzed. Three new genes were found and named PKPI-B1, PKPI-B2, and PKPI-B10: these were represented by five, two, and seven clones, respectively. The remaining four clones corresponded to the formerly characterized PKPI-B9 gene. These data show that at least four PKPI-B encoding genes are harbored in the genome of potato cv. Istrinskii. Their analysis suggests that variability of PKPI-B encoding genes in potato is limited and could be explained by cross-hybridization events in the ancestor forms rather than by random mutagenesis.
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页码：292 / 299
页数：7
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