Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library

被引：0
|
作者：
Qian Zhang
Hui Xu
Jing Zhao
Runying Zeng
机构：
[1] University of Hawaii at Manoa,Department of Civil and Environmental Engineering
[2] SOA,Key Laboratory of Marine Genetic Resource
来源：
Biotechnology Letters | 2014年 / 36卷
关键词：
Characterization; Gene expression; Metagenomic library; Penicillin G acylase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
One clone (ACPGA001) exhibiting penicillin G acylase (PGA) activity was screened from a metagenomic library by using a medium containing penicillin G. A novel PGA gene from the inserted fragment of ACPGA001 was obtained by sequencing. The amino acid sequence of ACPGA001 PGA exhibited <33 % similarity to PGAs retrieved from GenBank. This gene was expressed in Escherichia coli M15 and the recombinant protein was purified and characterized. The ACPGA001 PGA exhibited a maximum activity at 60 °C and showed high activity at pH 4–10 with an optimum pH of 8.0. This enzyme was stable at 40 °C for 70 min with a half-life of 60 min at 55 °C. These beneficial characteristics of ACPGA001 PGA provide some advantages for the potential application of ACPGA001 PGA in industry.
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页码：617 / 625
页数：8
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