Carbonic anhydrase activity in halophilic anaerobes from soda lakes

被引：0
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作者：
A. V. Pitryuk
M. A. Pusheva
E. V. Kupriyanova
机构：
[1] Russian Academy of Sciences,Winogradsky Institute of Microbiology
[2] Russian Academy of Sciences,Institute of Plant Physiology
来源：
Microbiology | 2006年 / 75卷
关键词：
carbonic anhydrase; alkaliphiles; anaerobes; acetogens; sulfate-reducers;
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摘要：
The activity and cellular localization of carbonic anhydrase (CA) in two alkaliphilic anaerobes growing in soda lakes at pH 9–10 were studied. CA activity in the cell extracts of the acetogenic bacterium Natroniella acetigena was comparable to that of neutrophilic acetogens. Hydrogenotrophically grown cells of Desulfonatronum lacustre exhibited higher CA activity compared to the cells grown on medium with formate. High CA activity in the cytoplasmic fraction and the absence of high activity in the extracellular fraction were demonstrated. We propose that the cytoplasmic CA in alkaliphilic sulfate-reducers participates in conversion of bicarbonate to CO2, which is reduced in the cell to acetate via the acetyl-CoA pathway.
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页码：12 / 14
页数：2
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