In Vitro cell attachment and In Vivo tissue infiltration of porous PLLA/β-TCP/SA bone scaffolds

By combining Poly-L-lactide (PLLA) braids and freeze-dried beta tricalcium phosphate (ß-TCP)/sodium alginate (SA) sponges, this study proposes to produce bone scaffolds that facilitate cell infiltration. The structure of the bone scaffolds are evaluated by a hydrophilicity test, in vitro degradation test, and porosity measurement, after which MTT aßsay and in vivo animal study are performed to evaluate their cytotoxicity and cell infiltration, respectively. The test results show that a braiding structure has a positive effect on the hydrophilicity and cell attachment of PLLA braids. An environment containing calcium ions affects the cell viability. Osteo conductivity and osteo inductivity are both essential for the implants, and the test results show that the combination of ß-TCP influences the compactness of the bone formation and cell infiltration. This study provides systematic research based on the bone scaffolds which simultaneously accelerate cell attachment and infiltration.