Fusion tags to enhance heterologous protein expression

被引：0
|
作者：
Mi-Ran Ki
Seung Pil Pack
机构：
[1] Korea University,Department of Biotechnology and Bioinformatics
[2] Korea University,Institute of Industrial Technology
来源：
Applied Microbiology and Biotechnology | 2020年 / 104卷
关键词：
Heterologous protein expression; Expression-enhancing tag; Fusion tag; Protein tag; Peptide tag; Solubility tag; Aggregation-prone tag; Inclusion body;
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学科分类号：
摘要：
Escherichia coli is the most widely used heterologous protein expression system. However, this system remains a challenge due to the low solubility of proteins, insufficient yield, and inclusion body formation. Numerous approaches have sought to address these issues. The use of a fusion tag is one of the most powerful strategies for obtaining large amounts of heterologous protein in E. coli expression system. Here, recent advances in fusion tags that increase the expression of proteins are reviewed. In addition, proposed concepts for designing peptide tags to increase protein expression are discussed.
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页码：2411 / 2425
页数：14
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