The influence of LIF (Leukemia Inhibitory Factor) on the functional status of R1 mouse embryonic stem cells

被引：0
|
作者：
Lobanok E.S. [1 ]
Mezhevikina L.M. [2 ]
Belyanovich L.M. [1 ]
Petrova R.R. [2 ]
Vasilevich I.B. [1 ]
Volotovsky I.D. [1 ]
Fesenko E.E. [2 ]
机构：
[1] Institute of Cell Biophysics and Cell Technology, NAN Belarus, Minsk 220072
[2] Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290
来源：
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | 2009年 / 3卷 / 1期
关键词：
Cell cycle; Cytokine LIF; Embryonic stem cells; Proliferation;
D O I：
10.1134/S1990750809010077
中图分类号：
学科分类号：
摘要：
The influence of cytokine LIF (Leukemia Inhibitory Factor) on the viability, and proliferation of mouse embryonic stem cells (ESC) (R1 cell line) and their distribution by cell cycle stages has been investigated. LIF (5-20 ng/ml) increased growth of colonies and maintained high proliferative and pluripotent properties of R1 cells. LIF was also involved into the inhibition of spontaneous cell differentiation and apoptotic cell death; it also decreased the rations of S/G2 + M cell cycle and doubling-time of cell population. © Pleiades Publishing, Ltd. 2009.
引用
收藏
页码：54 / 57
页数：3
相关论文
共 50 条
  • [21] Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice
    Lee, Jae Hee
    Lee, Eun Ju
    Lee, Chae Hyun
    Park, Jun Hong
    Han, Jae Yong
    Lim, Jeong Mook
    FERTILITY AND STERILITY, 2009, 92 (03) : 1133 - 1140
  • [22] Leukemia inhibitory factor-dependent transcriptional activation in embryonic stem cells
    Boeuf, H
    Hauss, C
    DeGraeve, F
    Baran, N
    Kedinger, C
    JOURNAL OF CELL BIOLOGY, 1997, 138 (06): : 1207 - 1217
  • [23] The regulation of embryonic stem cell differentiation by leukaemia inhibitory factor (LIF)
    Murray, P
    Edgar, D
    DIFFERENTIATION, 2001, 68 (4-5) : 227 - 234
  • [24] Role of suppressors of cytokine signaling (Socs) in leukemia inhibitory factor (LIF) -dependent embryonic stem cell survival
    Duval, D
    Reinhardt, S
    Kedinger, C
    Boeuf, H
    FASEB JOURNAL, 2000, 14 (11): : 1577 - 1584
  • [25] Leukemia Inhibitory Factor (LIF) overexpression increases angiogenic potential of mouse derived bone marrow mesenchymal stem/stromal cells
    Silva, D. N.
    Santos, G. C.
    Orge, I. D.
    Sampaio, G. A.
    Silveira, B. M.
    Paredes, B. D.
    Fortuna, V. A.
    Soares, M. B.
    CYTOTHERAPY, 2020, 22 (05) : S104 - S104
  • [26] Chromatin-related proteins in pluripotent mouse embryonic stem cells are downregulated after removal of leukemia inhibitory factor
    Kurisaki, A
    Hamazaki, TS
    Okabayashi, K
    Iida, T
    Nishine, T
    Chonan, R
    Kido, H
    Tsunasawa, S
    Nishimura, O
    Asashima, M
    Sugino, H
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 335 (03) : 667 - 675
  • [27] Leukemia Inhibitory Factor Promotes Hsp90 Association with STAT3 in Mouse Embryonic Stem Cells
    Setati, Mokgadi M.
    Prinsloo, Earl
    Longshaw, Victoria M.
    Murray, Patricia A.
    Edgar, David H.
    Blatch, Gregory L.
    IUBMB LIFE, 2010, 62 (01) : 61 - 66
  • [28] The Role of the Leukemia Inhibitory Factor (LIF) - Pathway in Derivation and Maintenance of Murine Pluripotent Stem Cells
    Graf, Urs
    Casanova, Elisa A.
    Cinelli, Paolo
    GENES, 2011, 2 (01) : 280 - 297
  • [29] In Vivo Gene Transfer of Leukemia Inhibitory Factor (LIF) into Mouse Endometrium
    Yao-Yuan Hsieh
    Chich-Sheng Lin
    Yu-Ling Sun
    Chi-Chen Chang
    Horng-Der Tsai
    Jackson Chieh-Hsi Wu
    Journal of Assisted Reproduction and Genetics, 2002, 19 : 79 - 83
  • [30] In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium
    Hsieh, YY
    Lin, CS
    Sun, YL
    Chang, CC
    Tsai, HD
    Wu, JCH
    JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 2002, 19 (02) : 79 - 83
12345