The effect of in planta TIBA and proline treatment on somatic embryogenesis of sugar beet (Beta vulgaris L.)

The effect of in planta TIBA and L-proline on in vitro seedlings and cell culture of sugar beet was investigated. Sterilized seeds were grown in vitro on 1/2 MS medium supplemented with 0 or 3 mg/l TIBA. Calli obtained on young leaves cultured on MS medium containing 1 mg/l BAP, were used for the initiation of cell suspension cultures using MS basal composition supplemented with 0 or 50 mM proline. Aliquots of 1 ml from cell suspension culture were inoculated onto the first somatic embryo induction MS medium containing TIBA 0.5 mg/l, BAP 1.0 mg/l, and proline at 0 or 50 mM. After three weeks of culture, embryogenic calli were transferred to the second embryo induction medium supplemented with NAA and BAP at 0.2 and 0.5 mg/l, respectively. The frequency of somatic embryos of calli obtained from in planta TIBA together with proline treatments on average was 20 which was higher than that of the other treatments.