Development and validation of an reversed-phase liquid chromatographic method for analysis of spiramycin and related substances

被引：0
|
作者：
H. K. Chepkwony
A. Vermaelen
E. Roets
J. Hoogmartens
机构：
[1] Universiteit Leuven,Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke
来源：
Chromatographia | 2001年 / 54卷
关键词：
Column liquid chromatography; Spiramycin; Factorial design; Robustness;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A simple, robust, sensitive isocratic liquid chromatographic (LC) method suitable for the analysis of spiramycin is described. This method utilizes XTerra RP18 as the stationary phase and acetonitrile-0.2m K2HPO4 (pH 6.5)-water, 39.5:5:55.5 (v/v), as mobile phase. The mobile phase flow rate is 1.0 mL min−1; the column is maintained at 70°C by immersion in a waterbath; and UV detection is performed at 232 nm. The method has good selectivity towards the major components (spiramycins I, II, and III), other related substances, and many impurities. The robustness of the method was evaluated by means of full-factorial experimental design.
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页码：51 / 56
页数：5
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