Production and characterization of interspecific hybrids between Brassica maurorum and crop brassicas

被引:0
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作者
B. Chrungu
N. Verma
A. Mohanty
A. Pradhan
K. R. Shivanna
机构
[1] Department of Botany,
[2] University of Delhi,undefined
[3] Delhi 110 007,undefined
[4] India,undefined
[5] National Bureau of Plant Genetic Resources,undefined
[6] Indian Agricultural Research Institute Campus,undefined
[7] New Delhi 110 012,undefined
[8] India,undefined
[9] Centre for Genetic Manipulation of Crop Plants,undefined
[10] Department of Genetics,undefined
[11] University of Delhi,undefined
[12] South Campus,undefined
[13] New Delhi 110 021,undefined
[14] India,undefined
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Key words Brassica maurorum; Crop brassicas; Embryo rescue; Wide hybrids; Amphidiploids;
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摘要
 Seven interspecific hybrids were produced between Brassica maurorum (♀), a wild species resistant to Alternaria blight and white rust, and all the monogenomic (B. campestris, B. nigra and B. oleracea) and digenomic (B. juncea, B. napus and B. oleracea) crop brassicas (♂) through embryo rescue. The hybrids were confirmed by means of morphological and cytological studies. All the hybrids were pollen-sterile. Amphidiploids were induced in three of the hybrids: B. maurorum×B. napus, B. maurorum×B. carinata, B. maurorum×B. nigra. The hybrids were also confirmed through DNA analyses for nuclear and organelle genomes using RAPD and RFLP techniques.
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页码:608 / 613
页数:5
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