Development of novel media formulation using rice straw and corn steep liquor for pyruvate production

Pyruvate was produced using novel organic macronutrients, pre-treated rice straw and corn steep (CSL) in the media formulation. The results were optimised using response surface methodology and compared using glycerol and jackfruit peel as carbon source. The compromised optimisation of process parameters with rice straw as carbon source reveals significant effects on pyruvate concentration influencing linearly at confidence level at less than 1%. Optimal levels of CSL concentration assisted with incubation time and temperature for glycerol, rice straw, and jackfruit peel resulted in an overall yield of 45.03 g/L, 41.94 g/L, and 40.57 g/L for pyruvate concentration and 0.36 g/L, 0.74 g/L, and 0.87 g/L for reducing sugars, respectively. The purified pyruvate salt extracted from the optimised runs of the three carbon sources was tested using SEM analysis and was found comparable to industrial product.