N-(2-hydroxy phenyl) acetamide: a novel suppressor of Toll-like receptors (TLR-2 and TLR-4) in adjuvant-induced arthritic rats

被引:0
|
作者
Kahkashan Perveen
Farina Hanif
Huma Jawed
Siddiqua Jamall
Shabana Usman Simjee
机构
[1] University of Karachi,Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences
[2] University of Karachi,H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences
[3] University of Karachi,Department of Biochemistry
来源
Molecular and Cellular Biochemistry | 2014年 / 394卷
关键词
Toll-like receptor; Tumor necrosis factor; Interleukin-1; Inflammation; N-(2-hydroxy phenyl) acetamide; Rheumatoid arthritis;
D O I
暂无
中图分类号
学科分类号
摘要
Toll-like receptors (TLRs) are key recognition structures of immune system and recently emerged as potential contributors to the inflammation observed in human and rodent models of arthritis. Present study aims to investigate the effect of N-(2-hydroxy phenyl)-acetamide (NA-2) on modulation of TLRs in the development of adjuvant-induced arthritis. Arthritis was induced by intradermal administration of heat-killed Mycobacterium tuberculosis H37Ra. The treatment of NA-2 (5 mg/kg) and indomethacin (5 mg/kg) was started in their respective group on the day of arthritis induction. Body weights, paw volume measurements, and nociception sensation (Plantar test) were done on alternate days to monitor the progression of the disease until arthritis score of four was observed in arthritic control group. Along with the clinical signs, histopathology of knee joints was also performed. The splenocytes cultures were prepared from each group; TLR-2 and TLR-4 mRNAs were analyzed in 48-h cultured splenocytes using RT-PCR; and the supernatants were used to determine IL-1β and TNF-α by ELISA. A significant reversal of deficit seen in body weights of the arthritic control group was observed in NA-2-treated animals with a parallel decrease in paw edema and transmission of nociception. Remission of the clinical signs and nociception was associated with improved histology. Compared with arthritic control, NA-2 treatment significantly decreased the level of IL-1β (p < 0.003) and TNF-α (p < 0.001) in the supernatants of cultured splenocytes. Likewise, NA-2 also reduced the expression of TLRs mRNA. Our findings suggest that NA-2 affects AIA in a pleiotropic manner, suppressing TLRs-mediated joint inflammation and related symptoms.
引用
收藏
页码:67 / 75
页数:8
相关论文
共 50 条
  • [31] Endogenous ligands for toll-like receptor (TLR)-4 but not TLR-2 are increased in serum of patients with systemic sclerosis
    Radstake, TRDJ
    Van Lieshout, TAWT
    Vonk, MC
    Roelofs, MF
    Van den Berg, WB
    Van den Hoogen, FHJ
    ANNALS OF THE RHEUMATIC DISEASES, 2005, 64 : 276 - 276
  • [32] Stimulants of Toll-like receptor (TLR)-2 and TLR-4 are abundant in certain minimally-processed vegetables
    Erridge, Clett
    FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (06) : 1464 - 1467
  • [33] The lipopolysaccharide-activated toll-like receptor (TLR)-4 induces synthesis of the closely related receptor TLR-2 in adipocytes
    Lin, Y
    Lee, H
    Berg, AH
    Lisanti, MP
    Shapiro, L
    Scherer, PE
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (32) : 24255 - 24263
  • [34] Expression of Toll-Like Receptors (TLR2 and TLR4) in the Eyes of Mice with Disseminated Acanthamoebiasis
    Kot, Karolina
    Kosik-Bogacka, Danuta
    Lanocha-Arendarczyk, Natalia
    Wojtkowiak-Giera, Agnieszka
    Kolasa-Wolosiuk, Agnieszka
    BIOMED RESEARCH INTERNATIONAL, 2019, 2019
  • [35] Acanthamoeba infection in lungs of mice expressed by toll-like receptors (TLR2 and TLR4)
    Derda, Monika
    Wojtkowiak-Giera, Agnieszka
    Kolasa-Wolosiuk, Agnieszka
    Kosik-Bogacka, Danuta
    Hadas, Edward
    Jagodzinski, Pawel P.
    Wandurska-Nowak, Elzbieta
    EXPERIMENTAL PARASITOLOGY, 2016, 165 : 30 - 34
  • [36] Shift From Toll-like Receptor 2 (TLR-2) Toward TLR-4 Dependency in the Erosive Stage of Chronic Streptococcal Cell Wall Arthritis Coincident With TLR-4-Mediated Interleukin-17 Production
    Abdollahi-Roodsaz, Shahla
    Joosten, Leo A. B.
    Helsen, Monique M.
    Walgreen, Birgitte
    van Lent, Peter L.
    van den Bersselaar, Liduine A.
    Koenders, Marije I.
    van den Berg, Wim B.
    ARTHRITIS AND RHEUMATISM, 2008, 58 (12): : 3753 - 3764
  • [37] Active Hexose Correlated Compound (AHCC) promotes an intestinal immune response in BALB/c mice and in primary intestinal epithelial cell culture involving toll-like receptors TLR-2 and TLR-4
    Mallet, Jean-Francois
    Graham, Emilie
    Ritz, Barry W.
    Homma, Kohei
    Matar, Chantal
    EUROPEAN JOURNAL OF NUTRITION, 2016, 55 (01) : 139 - 146
  • [38] Toll-like receptor (TLR)-2 and TLR-4 regulate inflammation, gliosis, and myelin sparing after spinal cord injury
    Kigerl, Kristina A.
    Lai, Wenmin
    Rivest, Serge
    Hart, Ronald P.
    Satoskar, Abhay R.
    Popovich, Phillip G.
    JOURNAL OF NEUROCHEMISTRY, 2007, 102 (01) : 37 - 50
  • [39] Active Hexose Correlated Compound (AHCC) promotes an intestinal immune response in BALB/c mice and in primary intestinal epithelial cell culture involving toll-like receptors TLR-2 and TLR-4
    Jean-François Mallet
    Émilie Graham
    Barry W. Ritz
    Kohei Homma
    Chantal Matar
    European Journal of Nutrition, 2016, 55 : 139 - 146
  • [40] Toll-like receptors TLR2 and TLR4 block the replication of pancreatic β cells in diet-induced obesity
    Ji, Yewei
    Sun, Shengyi
    Shrestha, Neha
    Darragh, Laurel B.
    Shirakawa, Jun
    Xing, Yuan
    He, Yi
    Carboneau, Bethany A.
    Kim, Hana
    An, Duo
    Ma, Minglin
    Oberholzer, Jose
    Soleimanpour, Scott A.
    Gannon, Maureen
    Liu, Chengyang
    Naji, Ali
    Kulkarni, Rohit N.
    Wang, Yong
    Kersten, Sander
    Qi, Ling
    NATURE IMMUNOLOGY, 2019, 20 (06) : 677 - +