Construction of a gene of the human tumor-associated antigen VNTR(MUC1) bound to streptavidin, its expression in Escherichia coli, and the study of properties of the hybrid protein

被引：0
|
作者：
Gul'ko L.B. [1 ]
Pavlova O.V. [1 ]
D'yakov N.A. [1 ]
Okorokova N.A. [1 ]
Ratmanova K.I. [1 ]
Logunova N.N. [1 ]
Bobreneva R.A. [1 ]
Makarov V.A. [1 ]
Yurin V.L. [1 ]
Veiko V.P. [1 ]
Debabov V.G. [1 ]
机构：
[1] State Res. Center of Genetics and Selection of Industrial Microorganisms of the Russian Federation, Pervyi Dorozhnyi proezd 1, Moscow
来源：
Russian Journal of Bioorganic Chemistry | 2000年 / 26卷 / 6期
关键词：
Escherichia coli; Hybrid protein; MUC1; Streptavidin; Tumor-associated antigen; VNTR;
D O I：
10.1007/BF02758665
中图分类号：
学科分类号：
摘要：
A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures effective secretion of the hybrid protein into the periplasmic space of Escherichia coli cells. The hybrid protein was isolated in a homogeneous state and its immunogenic properties were studied. © 2000 MAIK "Nauka/Interperiodica".
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页码：381 / 389
页数：8
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