Construction of a gene of the human tumor-associated antigen VNTR(MUC1) bound to streptavidin, its expression in Escherichia coli, and the study of properties of the hybrid protein

被引：0

作者：

Gul'ko, LB ^{[1
]}

Pavlova, OV ^{[1
]}

D'yakov, NA ^{[1
]}

Okorokova, NA ^{[1
]}

Ratmanova, KI ^{[1
]}

Logunova, NN ^{[1
]}

Bobreneva, RA ^{[1
]}

Makarov, VA ^{[1
]}

Yurin, VL ^{[1
]}

Veiko, VP ^{[1
]}

Debabov, VG ^{[1
]}

机构：

[1] State Res Ctr Genet & Select Ind Microorganisms R, Moscow 113545, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 2000年 / 26卷 / 06期

关键词：

Escherichia coli; hybrid protein; MUC1; streptavidin; tumor-associated antigen; VNTR;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures an effective secretion of the hybrid protein into the periplasmic space of Escherichia coli cells. The hybrid protein was isolated in a homogeneous state and its immunogenic properties were studied.

引用

页码：423 / 432

页数：10

共 19 条

[1] Construction of a gene of the human tumor-associated antigen VNTR(MUC1) bound to streptavidin, its expression in Escherichia coli, and the study of properties of the hybrid protein
Gul'ko L.B.
Pavlova O.V.
D'yakov N.A.
Okorokova N.A.
Ratmanova K.I.
Logunova N.N.
Bobreneva R.A.
Makarov V.A.
Yurin V.L.
Veiko V.P.
Debabov V.G.
Russian Journal of Bioorganic Chemistry, 2000, 26 (6) : 381 - 389
[2] Retargeting of human T cells to tumor-associated MUC1: The evolution of a chimeric antigen receptor
Wilkie, Scott
Picco, Gianfranco
Foster, Julie
Davies, David M.
Julien, Sylvain
Cooper, Lucienne
Arif, Sefina
Mather, Stephen J.
Taylor-Papadimitriou, Joyce
Burchell, Joy M.
Maher, John
JOURNAL OF IMMUNOLOGY, 2008, 180 (07): : 4901 - 4909
[3] Correlation of MUC1 tumor-associated antigen with growth factor receptors and VEGF expression in ovarian cancer
Budiu, Raluca
Mantia-Smaldone, Gina
Edwards, Robert
Vlad, Anda
CANCER RESEARCH, 2009, 69
[4] Evaluation of Tumor-Associated Antigen MUC1 Expression with the MACSima™ High-Content Imaging Platform
Herbel, Christoph
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Kuester, Laura N.
Schaefer, Daniel
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Hardt, Olaf
Eckardt, Dominik
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MOLECULAR THERAPY, 2020, 28 (04) : 149 - 149
[5] Preparation and evaluation of a tumor-associated antigen mucin (MUC1) and tumor-associated monoclonal antibody-derived hybrid peptide as a breast cancer imaging agent
Okarvi, Subhani
JOURNAL OF NUCLEAR MEDICINE, 2010, 51
[6] Thermodynamic switch in binding of human galectin-3 to tumor-associated TF antigen (CD176) and MUC1 glycopeptides
Cudic, Mare
Rodriguez, Maria C.
Yegorova, Svetlana
Pitteloud, Jean-Philippe
Chavaroche, Anais E.
Andre, Sabine
Arda, Ana
Minond, Dimitriy
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Gabius, Hans-Joachim
GLYCOBIOLOGY, 2015, 25 (11) : 1252 - 1253
[7] Design of Chimeric Proteins on the Basis of a Pentameric Superhelical Fragment of Human Cartilage Oligomeric Matrix Protein: II. The Properties of a Hybrid Containing the Antigen VNTR (MUC1) from Human Tumors
S. Yu. Belitskaya
E. F. Boldyreva
V. G. Korobko
Russian Journal of Bioorganic Chemistry, 2004, 30 : 42 - 46
[8] Design of chimeric proteins on the basis of a pentameric superhelical fragment of human cartilage oligomeric matrix protein: II. The properties of a hybrid containing the antigen VNTR (MUC1) from human tumors
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RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2004, 30 (01) : 42 - 46
[9] Protein and gene expression of tumor-associated antigen RCAS1 in esophageal squamous cell carcinoma
Ikeguchi, M
Ohoro, S
Maeda, Y
Yamaguchi, KI
Fukuda, K
Shirai, H
Kondo, A
Tsujitani, SI
Kaibara, N
ONCOLOGY REPORTS, 2003, 10 (06) : 1891 - 1894
[10] Expression of tumor-associated differentiation antigens, MUC1 glycoforms and CEA, in human thymic epithelial cells: Implications for self-tolerance and tumor therapy
Cloosen, Silvie
Arnold, Janna
Thio, Marco
Bos, Gerard M. J.
Kyewski, Bruno
Germeraad, Wilfred T. V.
CANCER RESEARCH, 2007, 67 (08) : 3919 - 3926

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