Thermal motions quantification of lysozyme in aqueous mixtures with different co-solvent: glycerol [C3H5(OH)3], trehalose [C12H22O11], sucrose [C12H22O11], and a chaotropic agent [CO(NH2)2] by osiris near-backscattering crystal analyser spectrometer

被引：0

作者：

G. Romeo

机构：

[1] Università di Messina,Dipartimento di Fisica and INFM

来源：

Applied Physics A | 2012年 / 106卷

关键词：

Lysozyme; Trehalose; Dynamical Structure Factor; Conformational Substrate; Methyl Group Rotation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Thermal neutron scattering is a powerful tool to directly probe protein internal motions. Here, we report an incoherent neutron scattering study of the effect of glycerol-, disaccharide- CO(NH2)2-aqueous mixtures on the internal dynamics of lysozyme powder. Mean-square displacement of amplitude motions as a function of temperature was evaluated. We showed the complex interdependence between the motions of the protein, water of hydration, and co-solvent. Analysis of data gives access to the geometry of the observed motions. Then the crucial role of the solvent mobility to activate thermal fluctuations is proposed.

引用

页码：893 / 900

页数：7

共 35 条

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