Thermal motions quantification of lysozyme in aqueous mixtures with different co-solvent: glycerol [C3H5(OH)3], trehalose [C12H22O11], sucrose [C12H22O11], and a chaotropic agent [CO(NH2)2] by osiris near-backscattering crystal analyser spectrometer

被引：0
|
作者：
G. Romeo
机构：
[1] Università di Messina,Dipartimento di Fisica and INFM
来源：
Applied Physics A | 2012年 / 106卷
关键词：
Lysozyme; Trehalose; Dynamical Structure Factor; Conformational Substrate; Methyl Group Rotation;
D O I：
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中图分类号：
学科分类号：
摘要：
Thermal neutron scattering is a powerful tool to directly probe protein internal motions. Here, we report an incoherent neutron scattering study of the effect of glycerol-, disaccharide- CO(NH2)2-aqueous mixtures on the internal dynamics of lysozyme powder. Mean-square displacement of amplitude motions as a function of temperature was evaluated. We showed the complex interdependence between the motions of the protein, water of hydration, and co-solvent. Analysis of data gives access to the geometry of the observed motions. Then the crucial role of the solvent mobility to activate thermal fluctuations is proposed.
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页码：893 / 900
页数：7
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