Two pathways of plant regeneration in wheat anther culture

被引:1
|
作者
R. Konieczny
A.Z. Czaplicki
H. Golczyk
L. Przywara
机构
[1] Jagiellonian University,Department of Plant Cytology and Embryology
[2] Plant Breeding and Acclimatization Institute,undefined
来源
关键词
Androgenesis; Anther culture; Gramineae; Organogenesis; Plant regeneration;
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摘要
The anthers of 10 Polish winter wheat (Triticum aestivum L.) cultivars were used for the induction of androgenesis and plant regeneration. The highest rate of callus induction (9.1%) and green plant production (0.8%) was obtained with the cultivar Apollo that was chosen for histological analysis. The first androgenic division was symmetrical and occurred after 3 weeks of culture. Further divisions of newly formed cells gave rise to multicellular structures which followed two developmental pathways: callus production or direct embryo formation. Plant regeneration was observed in both pathways. Chromosome counting of plantlets regenerated showed that haploid metaphases 2n=3x=21 were the most frequent.
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页码:177 / 187
页数:10
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