Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.

A taxonomic study was carried out on strain SA5d-4T, which was isolated from a marine limpet (Patelloida saccharina lanx [Reeve, 1855]) collected from intertidal rocks in Xiamen, China. Strain SA5d-4T was aerobic, Gram-positive, lacked flagellum, non-motile, filamentous, formed a slightly-yellowish colony, and non-sporulating. The strain grew optimally at 28 °C, at pH values 7.0–8.0, and in the presence of 1–2% (w/v) sodium chloride. The major cellular fatty acids identified were iso-C15:0, iso-C17:0ω10c, and iso-C17:0. The dominated respiratory quinone was menaquinone-7. The major phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G + C content was 35.3 mol%, calculated from a draft genome sequence. Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that strain SA5d-4T belongs to a new genus within the family Bacillaceae, and this gene shares 95.6% similarity with that from Bacillus taeanensis BH030017T, 95.2% with Bacillus algicola KMM 3737T, 95.1% with Bacillus alkalinitrilicus ANL-iso4T, 94.9% with Bacillus hwajinpoensis SW-72T, and 94.6% with Anaerobacillus alkalidiazotrophicus MS6T. Whole genome phylogenetic analyses indicated that strain SA5d-4T formed a monophyletic branch with B. taeanensis BH030017T. The average nucleotide identity between strain SA5d-4T and B. taeanensis BH030017T was 69.6%. Based on polyphasic taxonomic characteristics, strain SA5d-4T represents a novel species of a new genus, for which the name Lottiidibacillus patelloidae gen. nov., sp. nov., is proposed with the type strain SA5d-4T (= MCCC 1A11654T = KCTC 33831T). Based on phylogenetic analyses, B. taeanensis should be transferred to a new genus, named Maribacillus, as Maribacillus taeanensis comb. nov., with type strain BH030017T (= KCTC 3918T = DSM 16466T).