Fabrication of a paper-based analytical device for multiple colorimetric analysis via inkjet-printing and paper-cutting

We introduce a process for the fabrication of a paper-based analytical device (PAD) for multiple colorimetric analysis via paper- cutting and inkjet-printing. To create the selective flow channels, hydrophobic barriers were formed by using hydrophobic polyvinyl chloride tape-cutting and ultraviolet-curable resin printing. Multiple paper channels were isolated by the hydrophobic barriers on a single chip. An assay model was created from bovine serum albumin (BSA) and tetrabromophenol blue (TBPB) to verify the feasibility of the fabricated PAD. A multiple colorimetric analysis was demonstrated a reaction with different BSA concentrations after the TBPB regent was elaborately deposited onto the circular test spot areas by inkjet printing.