Genetic variability measures among Bromus catharticus Vahl. populations and cultivars with RAPD and AFLP markers

被引:0
|
作者
Débora I. Puecher
Claudio G. Robredo
Raúl D. Rios
Pedro Rimieri
机构
[1] CNIA-INTA,Instituto de Genética
[2] INTA-Pergamino,undefined
来源
Euphytica | 2001年 / 121卷
关键词
AFLPs; bulk DNA; genetic variability; prairie grass; RAPDs;
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中图分类号
学科分类号
摘要
The objectives of this study were to optimize RAPD and AFLP techniques in B. catharticus, and to determine the genetic variability of populations and commercial prairie grass cultivars with the aforementioned molecular markers. Two populations with contrasting morphological characteristics were evaluated from individual and bulked DNA samples using RAPD markers. Both analyses showed a similar information about inter population variability. Each accession was sampled by a single leaf bulk of 10 plants. Accession similarities were established with 276 RAPD and 714 AFLP bands using Jaccard similarity coefficient. The dendrogram of the accessions using RAPD markers showed that they shared high similarity values (>94%). A similar result was obtained with AFLP markers (similarity values >98%), revealing a narrow genetic basis in the analyzed accessions. Consequently, molecular characterization of germplasm should be considered in addition to morphological criteria, to choose the parental genotypes for breeding programs of this forage crop. The AFLP technique was more efficient to detect DNA polymorphism in our experiments and unique fingerprints were detected for all the accessions. RAPD is a simple and non expensive technique, suitable to estimate genetic similarity.
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页码:229 / 236
页数:7
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