Enhancement of DHA production from Aurantiochytrium sp. by atmospheric and room temperature plasma mutagenesis aided with microbial microdroplet culture screening

被引:0
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作者
Qing Wang
Wenbiao Jin
Wei Han
Kang Song
Yidi Chen
Chuan Chen
Guangming Jiang
Xu Zhou
机构
[1] Harbin Institute of Technology Shenzhen,State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering
[2] Harbin Institute of Technology (Shenzhen),Shenzhen Engineering Laboratory of Microalgae Bioenergy
[3] Chinese Academy of Sciences,State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology
[4] Harbin Institute of Technology,State Key Laboratory of Urban Water Resource and Environment, School of Environment
[5] University of Wollongong,School of Civil, Mining and Environmental Engineering
来源
关键词
sp.; DHA; Atmospheric and room temperature plasma mutagenesis; Microbial microdroplet culture system; Food waste liquid;
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学科分类号
摘要
ARTP mutagenesis aided MMC screening which was used for Aurantiochytrium sp. breeding.Optimal mutant strain obtained 9.22 g/L DHA yield and improved by 83.2%.The ratio of DHA in total fatty acids of mutant MA20 increased from 37.2 to 58.3%.Adding food waste liquid instead of yeast extract reduced 45% of the medium cost.
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页码:16807 / 16818
页数:11
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