HPLC determination of tranexamic acid in pharmaceutical preparations and blood

Tranexamic acid (Tr) reacts with 2-hydroxynaphthaldehyde (HN) in aqueous ethanol at neutral pH in a 1∶1 molar ratio to form a tranexamic acid-2-hydroxynaphthaldehyde (Tr-HN) derivative, with enhancement of spectrophotometric sensitivity. Tr-HN was eluted from a YMC-ODS column (150×4.6 mm id) and separated completely from the derivatizing reagent HN. Tr-HN also was separated from paracetamol and mefanemic acid. Quantitation was at 317 nm. The method was used for the determination of Tr from pharmaceutical preparations and blood samples after 2–3 hrs of administration of the drug. A number of additives and amino acids did not affect the determination.