Exact quantification of gene amplification in archival tissue sections by laser-assisted microdissection and real-time PCR

被引:0
|
作者
U Lehmann
S Glöckner
W Kleeberger
R von Wasielewski
H Kreipe
机构
[1] Institute of Pathology,
[2] Medizinische Hochschule Hannover,undefined
关键词
Gene Amplification; Intratumor Heterogeneity; Exact Quantification; Bystander Cell; Immunohistochemical Labelling;
D O I
10.1186/bcr175
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 50 条
  • [31] Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples
    Toewe, Stefanie
    Kleineidam, Kristina
    Schloter, Michael
    JOURNAL OF MICROBIOLOGICAL METHODS, 2010, 82 (03) : 338 - 341
  • [32] Detection and quantification of Aspergillus westerdijkiae in coffee beans based on selective amplification of β-tubulin gene by using real-time PCR
    Morello, Luis Gustavo
    Sartori, Daniele
    de Oliveira Martinez, Andre Luiz
    Carneiro Vieira, Maria Lucia
    Taniwaki, Marta Hiromi
    Pelegrinelli Fungaro, Maria Helena
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 119 (03) : 270 - 276
  • [33] Quantification of algal endosymbionts (Symbiodinium) in coral tissue using real-time PCR
    Mieog, J. C.
    Van Oppen, M. J. H.
    Berkelmans, R.
    Stam, W. T.
    Olsen, J. L.
    MOLECULAR ECOLOGY RESOURCES, 2009, 9 (01) : 74 - 82
  • [34] Absolute quantification of gene expression in biomaterials research using real-time PCR
    Leong, David Tai
    Gupta, Anurag
    Bai, Hui Fen
    Wan, Guoqiang
    Yoong, Li Foong
    Too, Heng-Phon
    Chew, Fook Tim
    Hutmacher, Dietmar Werner
    BIOMATERIALS, 2007, 28 (02) : 203 - 210
  • [35] Robust quantification of the SMN gene copy number by real-time TaqMan PCR
    Ilsa Gómez-Curet
    Karyn G. Robinson
    Vicky L. Funanage
    Thomas O. Crawford
    Mena Scavina
    Wenlan Wang
    neurogenetics, 2009, 10 : 171 - 172
  • [36] Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR
    Henry, S
    Baudoin, E
    López-Gutiérrez, JC
    Martin-Laurent, F
    Brauman, A
    Philippot, L
    JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (03) : 327 - 335
  • [37] Robust quantification of the SMN gene copy number by real-time TaqMan PCR
    Ilsa Gómez-Curet
    Karyn G. Robinson
    Vicky L. Funanage
    Thomas O. Crawford
    Mena Scavina
    Wenlan Wang
    Neurogenetics, 2007, 8 : 271 - 278
  • [38] Comparison between quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for quantification of Leishmania parasites
    van der Meide, Wendy
    Guerra, Jorge
    Schoone, Gerard
    Farenhorst, Marit
    Coelho, Leila
    Faber, William
    Peekel, Inge
    Schallig, Henk
    JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) : 73 - 78
  • [39] Robust quantification of the SMN gene copy number by real-time TaqMan PCR
    Gomez-Curet, Ilsa
    Robinson, Karyn G.
    Funanage, Vicky L.
    Crawford, Thomas O.
    Scavina, Mena
    Wang, Wenlan
    NEUROGENETICS, 2007, 8 (04) : 271 - 278
  • [40] Quantitative real-time PCR assisted cell counting (qPACC) for epigenetic - based immune cell quantification in blood and tissue
    Kleen, Thomas Oliver
    Yuan, Jianda
    JOURNAL FOR IMMUNOTHERAPY OF CANCER, 2015, 3