Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B

被引:0
|
作者
S. Zhou
L.P. Yomano
K.T. Shanmugam
L.O. Ingram
机构
[1] Northern Illinois University,Department of Biological Sciences
[2] University of Florida,Department of Microbiology and Cell Science
来源
Biotechnology Letters | 2005年 / 27卷
关键词
lactate; polylactic acid; polylactide; sucrose;
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学科分类号
摘要
Derivatives of ethanologenic Escherichia coli K011 were constructed for d(−)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based selection for mutants with improved performance. Resulting strains, SZ132 and SZ186, contain native genes for sucrose utilization. No foreign genes are present in SZ186. Strain SZ132 also contains a chromosomally integrated endoglucanase gene (Erwinia chrysanthemi celY). Strain SZ132 produced over 1 mol lactate per liter of complex medium containing 10% (w/v) sugar (fermentation times of 48 h for glucose, 120 h for sucrose). Both strains produced 667–700 mmol lactate per liter of mineral salts medium. Yields for metabolized sugar ranged from 88% to 95% in both media.
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页码:1891 / 1896
页数:5
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