Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

被引：0

作者：

Tuba Yasmin

Berlin D. Nelson

Houston A. Hobbs

Nancy K. McCoppin

Kris N. Lambert

Leslie L. Domier

机构：

[1] University of Illinois,Department of Crop Sciences

[2] North Dakota State University,Department of Plant Pathology

[3] United States Department of Agriculture,undefined

[4] Agricultural Research Service,undefined

来源：

Archives of Virology | 2017年 / 162卷

关键词：

Reverse Transcription Polymerase Chain Reaction; Predicted Amino Acid Sequence; Hepatitis Delta Virus; Ringspot Virus; Nontranslated Region;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3’ nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.

引用

页码：1089 / 1092

页数：3

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