Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

被引：0
|
作者：
Eva Spitalska
Alessandra Torina
Vincenza Cannella
Santo Caracappa
Olivier A. E. Sparagano
机构：
[1] University of Newcastle,School of Agriculture, Food and Rural Development
[2] Slovak Academy of Sciences,undefined
[3] Istituto Zooprofilattico Sperimentale della Sicilia,undefined
来源：
Parasitology Research | 2004年 / 94卷
关键词：
Restriction Fragment Length Polymorphism; Mixed Infection; Small Ruminant; Babesia; Ixodid Tick;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.
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页码：318 / 320
页数：2
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