Discrimination between Theileria lestoquardi (hirci) and Theileria annulata

被引:0
|
作者
Kirvar, E [1 ]
Katzer, F [1 ]
Ilhan, T [1 ]
Wilkie, GM [1 ]
Hooshmand-Rad, P [1 ]
Melrose, TD [1 ]
Brown, CGD [1 ]
机构
[1] Univ Edinburgh, Ctr Trop Vet Med, Roslin, Midlothian, Scotland
关键词
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中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A variety of biological and molecular biological tests were performed on the different life-cycle stages of several stocks of Theileria lestoquardi and T. annulata, the causative agents of two economically important diseases of sheep and cattle respectively. Analysis of the major merozoite/piroplasm surface antigen (mMPSA) gene and Restriction Fragment Length Polymorphisms (RFLP) of each species confirmed that the parasites were closely related. Infection of cattle, sheep and goats or their peripheral blood mononuclear cells (PBM) in vitro with sporozoites, isoenzyme analysis and Polymerase Chain Reaction (PCR) using sporozoites, schizonts and piroplasms of both species are tests that can be used to discriminate between them and thus contribute to knowledge on the epidemiology of both diseases.
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页码:303 / 310
页数:8
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