Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

被引：0
|
作者：
Martin D Witte
Tongfei Wu
Carla P Guimaraes
Christopher S Theile
Annet E M Blom
Jessica R Ingram
Zeyang Li
Lenka Kundrat
Shalom D Goldberg
Hidde L Ploegh
机构：
[1] Whitehead Institute for Biomedical Research,Department of Biology
[2] Cambridge,undefined
[3] Massachusetts,undefined
[4] USA.,undefined
[5] Oncology Medicinal Chemistry,undefined
[6] Janssen Research and Development,undefined
[7] Centyrex,undefined
[8] Janssen Research and Development,undefined
[9] LLC,undefined
[10] Massachusetts Institute of Technology,undefined
[11] Cambridge,undefined
[12] Massachusetts,undefined
[13] USA.,undefined
[14] Present address: Bio-Organic Chemistry,undefined
[15] Stratingh Institute for Chemistry,undefined
[16] University of Groningen,undefined
[17] Groningen,undefined
[18] the Netherlands.,undefined
来源：
Nature Protocols | 2015年 / 10卷
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学科分类号：
摘要：
Sortase A can be used for the site-specific modification of proteins. This protocol describes its immobilization on Sepharose beads, allowing for larger-scale continuous flow reactions or easy separation from the enzyme in batch reactions.
引用
收藏
页码：508 / 516
页数：8
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