Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

被引：0
|
作者：
Martin D Witte
Tongfei Wu
Carla P Guimaraes
Christopher S Theile
Annet E M Blom
Jessica R Ingram
Zeyang Li
Lenka Kundrat
Shalom D Goldberg
Hidde L Ploegh
机构：
[1] Whitehead Institute for Biomedical Research,Department of Biology
[2] Cambridge,undefined
[3] Massachusetts,undefined
[4] USA.,undefined
[5] Oncology Medicinal Chemistry,undefined
[6] Janssen Research and Development,undefined
[7] Centyrex,undefined
[8] Janssen Research and Development,undefined
[9] LLC,undefined
[10] Massachusetts Institute of Technology,undefined
[11] Cambridge,undefined
[12] Massachusetts,undefined
[13] USA.,undefined
[14] Present address: Bio-Organic Chemistry,undefined
[15] Stratingh Institute for Chemistry,undefined
[16] University of Groningen,undefined
[17] Groningen,undefined
[18] the Netherlands.,undefined
来源：
Nature Protocols | 2015年 / 10卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Sortase A can be used for the site-specific modification of proteins. This protocol describes its immobilization on Sepharose beads, allowing for larger-scale continuous flow reactions or easy separation from the enzyme in batch reactions.
引用
收藏
页码：508 / 516
页数：8
相关论文
共 50 条
  • [1] Site-specific protein modification using immobilized sortase in batch and continuous-flow systems
    Witte, Martin D.
    Wu, Tongfei
    Guimaraes, Carla P.
    Theile, Christopher S.
    Blom, Annet E. M.
    Ingram, Jessica R.
    Li, Zeyang
    Kundrat, Lenka
    Goldberg, Shalom D.
    Ploegh, Hidde L.
    NATURE PROTOCOLS, 2015, 10 (03) : 508 - 516
  • [2] Site-specific streptavidin modification using sortase
    Matsumoto, Takuya
    Sawamoto, Shiori
    Sakamoto, Takayuki
    Tanaka, Tsutomu
    Kondo, Akihiko
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2009, 108 : S108 - S108
  • [3] Site-specific protein modification on living cells catalyzed by Sortase
    Tanaka, Tsutomu
    Yamamoto, Teruyasu
    Tsukiji, Shinya
    Nagamune, Teruyuki
    CHEMBIOCHEM, 2008, 9 (05) : 802 - 807
  • [4] Challenges in the use of sortase and other peptide ligases for site-specific protein modification
    Morgan, Holly E.
    Turnbull, W. Bruce
    Webb, Michael E.
    CHEMICAL SOCIETY REVIEWS, 2022, 51 (10) : 4121 - 4145
  • [5] Site-specific tetrameric streptavidin-protein conjugation using sortase A
    Matsumoto, Takuya
    Sawamoto, Shiori
    Sakamoto, Takayuki
    Tanaka, Tsutomu
    Fukuda, Hideki
    Kondo, Akihiko
    JOURNAL OF BIOTECHNOLOGY, 2011, 152 (1-2) : 37 - 42
  • [6] Sortase-mediated ligations for the site-specific modification of proteins
    Schmohl, Lena
    Schwarzer, Dirk
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2014, 22 : 122 - 128
  • [7] Site-specific protein modification using a ketone handle
    Cornish, VW
    Hahn, KM
    Schultz, PG
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (34) : 8150 - 8151
  • [8] Sortase A-mediated site-specific labeling of Tau protein
    Li, Yuanjie
    Awasthi, Saurabh
    Bryan, Louise
    Nirmalraj, Peter N.
    Mayer, Michael
    BIOPHYSICAL JOURNAL, 2022, 121 (03) : 353A - 353A
  • [9] New Method for Site-Specific Modification of Liposomes with Proteins Using Sortase A-Mediated Transpeptidation
    Guo, Xueqing
    Wu, Zhimeng
    Guo, Zhongwu
    BIOCONJUGATE CHEMISTRY, 2012, 23 (03) : 650 - 655
  • [10] IMMOBILIZED BIOLUMINESCENT REAGENTS IN CONTINUOUS-FLOW SYSTEMS
    WORSFOLD, PJ
    NABI, A
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1986, 5 (07) : 183 - 185
12345