Biomimetic spinning of artificial spider silk from a chimeric minispidroin

被引：0

作者：

Andersson M. ^{[1
]}

Jia Q. ^{[2
]}

Abella A. ^{[3
]}

Lee X.-Y. ^{[3
]}

Landreh M. ^{[4
]}

Purhonen P. ^{[5
,6
]}

Hebert H. ^{[5
,6
]}

Tenje M. ^{[7
,8
]}

Robinson C.V. ^{[4
]}

Meng Q. ^{[2
]}

Plaza G.R. ^{[3
]}

Johansson J. ^{[1
,9
]}

Rising A. ^{[1
,9
]}

机构：

[1] Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala

[2] Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai

[3] ETSI de Caminos, Center for Biomedical Technology, Universidad Politécnica de Madrid, Madrid

[4] Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford

[5] Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm

[6] School of Technology and Health, KTH Royal Institute of Technology, Stockholm

[7] Department of Engineering Sciences, SciLifeLab, Uppsala University, Uppsala

[8] Department of Biomedical Engineering, Lund University, Lund

[9] Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet, Stockholm

来源：

Nature Chemical Biology | 2017年 / 13卷 / 3期

关键词：

D O I：

10.1038/nchembio.2269

中图分类号：

学科分类号：

摘要：

Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved.

引用

页码：262 / 264

页数：2

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