Prussian Blue Nanoparticle-Labeled Mesenchymal Stem Cells: Evaluation of Cell Viability, Proliferation, Migration, Differentiation, Cytoskeleton, and Protein Expression In Vitro

被引:0
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作者
Jirui Wen
Zhiwei Zhao
Ruijie Tong
Liwei Huang
Yali Miao
Jiang Wu
机构
[1] Sichuan University,West China School of Basic Medical Sciences & Forensic Medicine
[2] Shenyang University of Chemical Technology,College of Pharmaceutical and Biological Engineering
[3] Sichuan University,West China School of Stomatology Medicine
[4] Department of Gynecology,undefined
[5] West China Second University Hospital,undefined
[6] Sichuan University,undefined
[7] Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University),undefined
[8] Deep Undergroud Space Medical Center,undefined
[9] West China Hospital,undefined
[10] Sichuan University,undefined
来源
Nanoscale Research Letters | 2018年 / 13卷
关键词
Prussian blue nanoparticles; Mesenchymal stem cells; Magnetic resonance imaging; Cytotoxicity;
D O I
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中图分类号
学科分类号
摘要
Mesenchymal stem cells (MSCs) have been used for the treatment of various human diseases. To better understand the mechanism of this action and the fate of these cells, magnetic resonance imaging (MRI) has been used for the tracking of transplanted stem cells. Prussian blue nanoparticles (PBNPs) have been demonstrated to have the ability of labeling cells to visualize them as an effective MRI contrast agent. In this study, we aimed to investigate the efficiency and biological effects of labeled MSCs using PBNPs. We first synthesized and characterized the PBNPs. Then, iCELLigence real-time cell analysis system revealed that PBNPs did not significantly alter cell viability, proliferation, and migration activity in PBNP-labeled MSCs. Oil Red O staining and Alizarin Red staining revealed that labeled MSCs also have a normal differentiation capacity. Phalloidin staining showed no negative effect of PBNPs on the cytoskeleton. Western blot analysis indicated that PBNPs also did not change the expression of β-catenin and vimentin of MSCs. In vitro MRI, the pellets of the MSCs incubated with PBNPs showed a clear MRI signal darkening effect. In conclusion, PBNPs can be effectively used for the labeling of MSCs and will not influence the biological characteristics of MSCs.
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