Expression of a fusion protein of Pyrus pyrifolia S-RNase with glutathione-S-transferase in E. coli

被引：0

作者：

Niranjali Gamage

Takanori Matsuura

Naoko Norioka

Yumi Yoshimura

Takeshi Takasaki

Tetsu Nakanishi

Shigemi Norioka

机构：

[1] Kobe University,Faculty of Agriculture

[2] Osaka University,Institute for Protein Research

[3] Suita,undefined

来源：

Biotechnology Letters | 2000年 / 22卷

关键词：

fusion protein; glutathione-; -transferase; self-incompatibility; S-RNase;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

S-RNase is a style-specific ribonuclease which is associated with gametophytic self-incompatibility. An expression vector of a fusion protein of Pyrus pyrifolia(Japanese pear) S3-RNase with glutathione-S-transferase (GST) was constructed and transformed into E. coli. Using this system, the fusion protein, GST-S3-RNase, was expressed as an active form and can be used for screening pollen S-gene product(s).

引用

页码：1413 / 1417

页数：4

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