Bacillus cellulasensis sp. nov., isolated from marine sediment

A novel bacterial strain NIO-1130T was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130T showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387T (98.4 %) and Bacillus niabensis CIP 109816T (98.1 %), whereas other Bacillus species showed <97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA–DNA hybridization studies revealed that strain NIO-1130T exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387T and Bacillus niabensis CIP 109816T. On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130T (=NCIM 5461T=CCTCC AB 2011126T).