Adsorptive stripping square-wave voltammetric behavior of insulin

被引：0
|
作者：
Berzas Nevado J.J. [1 ]
Rodriguez Flores J. [1 ]
Castañeda Peñalvo G. [1 ]
机构：
[1] Departamento de Quimica Analítica y Tecnología de Alimentos, Facultad de Químicas, Universidad de Castilla-La Mancha
来源：
Fresenius' Journal of Analytical Chemistry | 1999年 / 364卷 / 8期
关键词：
Concentration Level; Human Insulin; Pharmaceutical Product; Analytical Methodology; Aqueous Sample;
D O I：
10.1007/s002160051427
中图分类号：
学科分类号：
摘要：
The adsorptive stripping voltammetric behavior of bovine insulin has been studied by both differential pulse and square-wave techniques, leading to analytical methodologies for its determination in aqueous samples and pharmaceutical preparations. The application of a square-wave mode for the stripping of adsorbed bovine insulin has proved to be much more sensitive, yielding signals 20 times larger than those obtained by applying a differential pulse scan. The precision obtained for the voltammetric method, at a concentration level as low as 0.77 mg L-1 of the protein, was found to be 1.45% (RSD., n = 8) by SWS and 2.20% (RSD., n = 8) by DPS. The pharmaceutical product Actrapid containing biosynthetic human insulin was analyzed by both methods. © Springer-Verlag 1999.
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页码：753 / 757
页数：4
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