Lipoplexes of dicationic gemini surfactants with DNA: Structural features of DNA compaction and transfection efficiency

被引：0
|
作者：
D. A. Faizullin
Yu. F. Zuev
L. Ya. Zakharova
A. G. Pokrovsky
V. A. Korobeinikov
T. A. Mukhametzyanov
A. I. Konovalov
机构：
[1] Kazan Scientific Center of the Russian Academy of Sciences,Kazan Institute of Biochemistry and Biophysics
[2] Russian Academy of Sciences,Arbuzov Institute of Organic and Physical Chemistry, Kazan Research Center
[3] Novosibirsk State University,undefined
[4] Kazan (Volga Region) Federal University,undefined
来源：
Doklady Biochemistry and Biophysics | 2015年 / 465卷
关键词：
Transfection Efficiency; Circular Dichroism Spectrum; HEK293T Cell; Gemini Surfac; DOKLADY Biochemistry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The internal structure of DNA lipoplexes with hydroxyethylated alkylammonium gemini surfactants (GS) with high transfection activity was studied by circular dichroism. It was shown that the efficiency of transfection of HEK293T cells with the pEGFP-N1 circular plasmid was different from zero only in the region of existence of chiral supramolecular DNA-GS complexes and reaches a maximum at concentrations at which the spontaneous aggregation of components is observed.
引用
收藏
页码：432 / 435
页数：3
相关论文
共 50 条
  • [1] Lipoplexes of dicationic gemini surfactants with DNA: Structural features of DNA compaction and transfection efficiency
    Faizullin, D. A.
    Zuev, Yu. F.
    Zakharova, L. Ya.
    Pokrovsky, A. G.
    Korobeinikov, V. A.
    Mukhametzyanov, T. A.
    Konovalov, A. I.
    DOKLADY BIOCHEMISTRY AND BIOPHYSICS, 2015, 465 (01) : 432 - 435
  • [2] Structural and spectroscopic studies on the formation of lipoplexes between DNA and cationic gemini surfactants
    Andrzejewska, Weronika
    Pietralik, Zuzanna
    Taube, Michal
    Skrzypczak, Andrzej
    Kozak, Maciej
    POLIMERY, 2014, 59 (7-8) : 569 - 574
  • [3] Compaction of DNA by gemini surfactants:: Effects of surfactant architecture
    Karlsson, L
    van Eijk, MCP
    Söderman, O
    JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2002, 252 (02) : 290 - 296
  • [4] DNA compaction to multi-molecular DNA condensation induced by cationic imidazolium gemini surfactants
    Zhou, Ting
    Xu, Guiying
    Ao, Mingqi
    Yang, Yanlian
    Wang, Chen
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2012, 414 : 33 - 40
  • [5] Dicationic Amino Substituted Gemini Surfactants and their Nanoplexes: Improved Synthesis and Characterization of Transfection Efficiency and Corneal Penetration In Vitro
    Lokesh Narsineni
    Marianna Foldvari
    Pharmaceutical Research, 2020, 37
  • [6] Dicationic Amino Substituted Gemini Surfactants and their Nanoplexes: Improved Synthesis and Characterization of Transfection Efficiency and Corneal PenetrationIn Vitro
    Narsineni, Lokesh
    Foldvari, Marianna
    PHARMACEUTICAL RESEARCH, 2020, 37 (07)
  • [7] PEGylated lipoplexes: Preparation Protocols affecting DNA condensation and cell Transfection efficiency
    Bombelli, Cecilia
    Faggioli, Francesca
    Luciani, Paola
    Mancini, Giovanna
    Sacco, Maria Grazia
    JOURNAL OF MEDICINAL CHEMISTRY, 2007, 50 (24) : 6274 - 6278
  • [8] DNA-DOPE-gemini surfactants complexes at low surface charge density: from structure to transfection efficiency
    Hubcik, Lukas
    Gallikova, Dominika
    Pullmannova, Petra
    Lacinova, L'ubica
    Sulova, Zdena
    Hanulova, Maria
    Funari, Sergio S.
    Devinsky, Ferdinand
    Uhrikova, Daniela
    GENERAL PHYSIOLOGY AND BIOPHYSICS, 2018, 37 (01) : 57 - 69
  • [9] Incorporation of gemini surfactants into pyridinium lipoplexes enhances transfection through endosomal membrane destabilization
    Sharma, Vishnu Dutt
    Ilies, Marc A.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014, 248
  • [10] Cell transfection by DNA-lipid complexes — Lipoplexes
    Y. S. Tarahovsky
    Biochemistry (Moscow), 2009, 74 : 1293 - 1304
12345