Structural and spectroscopic studies on the formation of lipoplexes between DNA and cationic gemini surfactants

被引：8
|
作者：
Andrzejewska, Weronika [1 ]
Pietralik, Zuzanna [1 ]
Taube, Michal [1 ]
Skrzypczak, Andrzej [2 ]
Kozak, Maciej [1 ]
机构：
[1] Adam Mickiewicz Univ, Dept Macromol Phys, Poznan, Poland
[2] Poznan Univ Tech, Fac Chem Technol, Poznan, Poland
来源：
POLIMERY | 2014年 / 59卷 / 7-8期
关键词：
gene therapy; gemini surfactants; DNA; circular dichroism; gel electrophoresis; COMPLEXES; SYSTEMS; TRANSFECTION; CARRIERS; CHLORIDE;
D O I：
10.14314/polimery.2014.569
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The process of complex formation between cationic gemini surfactants, 3,3'-[alpha,omega-(dioxaalkane)]bis(1-dodecylimidazolium) dichloride, with deoxyribonucleic acid (DNA) was studied. The study was performed for ten surfactants having spacer groups of different lengths used in 6 concentrations (5 mM, 2 mM, 1 mM, 0.5 mM, 0.2 mM, 0.1 mM) and a 6.5 mu M DNA solution. The complex formation was verified by circular dichroism spectroscopy and gel electrophoresis. The complexes were found to be stable and the process of complex formation was reproducible, efficient and immediate.
引用
收藏
页码：569 / 574
页数：6
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