Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels

被引：0
|
作者：
Yangyang Yan
Youshan Yang
Shumin Bian
Fred J. Sigworth
机构：
[1] Yale University,Department of Cellular and Molecular Physiology
来源：
The Journal of Membrane Biology | 2012年 / 245卷
关键词：
Artificial planar membrane; Ion channel expression and reconstitution; Patch clamp; Potassium ion channel;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na+-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.
引用
收藏
页码：667 / 674
页数：7
相关论文
共 50 条
  • [41] A SODIUM-ACTIVATED POTASSIUM CURRENT IN CULTURED BRAIN-STEM NEURONS
    DRYER, SE
    FUJII, J
    MARTIN, AR
    JOURNAL OF PHYSIOLOGY-LONDON, 1988, 398 : P12 - P12
  • [42] The sodium-activated potassium channel is encoded by a member of the Slo gene family
    Yuan, A
    Santi, CM
    Wei, A
    Wang, ZW
    Pollak, K
    Nonet, M
    Kaczmarek, L
    Crowder, CM
    Salkoff', L
    NEURON, 2003, 37 (05) : 765 - 773
  • [43] Sodium-activated K+ channels are also calcium-activated K+ channels.
    Qian, X
    Butler, A
    Salkoff, L
    Magleby, KL
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 119A - 119A
  • [44] RENAL SODIUM-ACTIVATED AND POTASSIUM-ACTIVATED ADENOSINE-TRIPHOSPHATASE AND SODIUM REABSORPTION IN HYPOTHYROID RAT
    KATZ, AI
    LINDHEIMER, MD
    JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (04): : 796 - 804
  • [45] ORGAN SPECIFICITY OF RAT SODIUM-ACTIVATED AND POTASSIUM-ACTIVATED ADENOSINE-TRIPHOSPHATASE
    URAYAMA, O
    NAKAO, M
    JOURNAL OF BIOCHEMISTRY, 1979, 86 (05): : 1371 - 1381
  • [46] SODIUM-ACTIVATED AND POTASSIUM-ACTIVATED ATPASE OF BEEF BRAIN - EFFECTS OF SOME TRANQUILIZERS
    UEDA, I
    WADA, T
    BALLINGER, CM
    BIOCHEMICAL PHARMACOLOGY, 1971, 20 (07) : 1697 - +
  • [47] International Union of Basic and Clinical Pharmacology. C. Nomenclature and Properties of Calcium-Activated and Sodium-Activated Potassium Channels
    Kaczmarek, Leonard K.
    Aldrich, Richard W.
    Chandy, K. George
    Grissmer, Stephan
    Wei, Aguan D.
    Wulff, Heike
    PHARMACOLOGICAL REVIEWS, 2017, 69 (01) : 1 - 11
  • [48] The Slack Sodium-Activated Potassium Channel Provides a Major Outward Current in Olfactory Neurons of Kv1.3-/- Super-Smeller Mice
    Lu, Songqing
    Das, Paromita
    Fadool, Debra A.
    Kaczmarek, Leonard K.
    JOURNAL OF NEUROPHYSIOLOGY, 2010, 103 (06) : 3311 - 3319
  • [49] FLUORESCENT DERIVATIVES OF STROPHANTHIDIN - INTERACTION WITH SODIUM-ACTIVATED AND POTASSIUM-ACTIVATED ADENOSINE-TRIPHOSPHATASE
    OESCH, F
    DALY, JW
    WATERS, JA
    WITKOP, B
    JOURNAL OF MEDICINAL CHEMISTRY, 1972, 15 (07) : 757 - &
  • [50] ALDOSTERONE ACTION AND SODIUM-ACTIVATED AND POTASSIUM-ACTIVATED ADENOSINE-TRIPHOSPHATASE IN TOAD BLADDER
    HILL, JH
    CORTAS, N
    WALSER, M
    JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (01): : 185 - 189
12345