Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels

被引：0
|
作者：
Yangyang Yan
Youshan Yang
Shumin Bian
Fred J. Sigworth
机构：
[1] Yale University,Department of Cellular and Molecular Physiology
来源：
The Journal of Membrane Biology | 2012年 / 245卷
关键词：
Artificial planar membrane; Ion channel expression and reconstitution; Patch clamp; Potassium ion channel;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na+-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.
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页码：667 / 674
页数：7
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