Determination of Fatty Acid Methyl Esters in Biodiesel Produced from Yellow Horn Oil by LC

A simple and accurate HPLC method with refractive index detection was developed to determine the main fatty acid methyl esters in biodiesel produced from yellow horn oil. Methyl linoleate, methyl linolenate, methyl arachidate, methyl stearate, methyl palmitate and methyl oleate were separated on a HIQ SIL C18W column using methanol as mobile phase. The method has good repeatability and precision, the intraday and interday RSD for both retention time and peak area was less than 3.2%. The LOD (S/N = 3) and LOQ (S/N = 9) were less than 0.004 and 0.015 mg mL−1, respectively.