Bead-to-bead transfer of Vero cells in microcarrier culture

被引:0
|
作者
Yushu Wang
Fan Ouyang
机构
[1] State Key Laboratory of Biochemical Engineering,
来源
Cytotechnology | 1999年 / 31卷
关键词
bead-to-bead transfer; microcarrier culture; recycled microcarrier; scale-up;
D O I
暂无
中图分类号
学科分类号
摘要
Cell harvesting technique is of considerable importance in the scale-up of microcarrier cultures of anchorage-dependent cells. The traditional methods are often time- and labor-consuming and cause physiological damage to the cells. Bead-to-bead cell transfer provides an attractive solution to the scale up process. By intermittent agitation, successful cell transfer was achieved. Significant cell growth was observed where bare beads contacted with confluent ones. Most of the fresh microcarriers reached near confluence four days after addition into the culture medium.
引用
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页码:221 / 224
页数:3
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