Immobilization of α-amylase in vinyl-polymer-based interpenetrating polymer networks

被引：0
|
作者：
A. K. Bajpai
S. Bhanu
机构：
[1] Government Autonomous Science College,Bose Memorial Research Laboratory, Department of Chemistry
来源：
Colloid and Polymer Science | 2003年 / 282卷
关键词：
Interpenetrating polymer network; α-Amylase; Immobilization; Kinetics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Semi-interpenetrating polymer networks (IPNs) of poly(ethylene glycol), poly(vinyl alcohol) and polyacrylamide were prepared as a support for enzyme immobilization and kinetic studies were performed for the immobilization of α-amylase. The effect of IPN composition on the extent of immobilization was investigated and the percentage of relative activity of the immobilized enzyme was evaluated as a function of the chemical architecture of the IPNs, pH and temperature, taking starch as a substrate. The kinetic constants and the maximum reaction velocity were also evaluated. The IPNs were characterized by IR spectral analysis.
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页码：76 / 83
页数：7
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