Screening of cry2 genes in Bacillus thuringiensis isolates from Argentina

被引:0
|
作者
Diego H. Sauka
Jorge G. Cozzi
Graciela B. Benintende
机构
[1] Instituto Nacional de Tecnología Agropecuaria (INTA),Area Bioinsumos Microbianos, Instituto de Microbiología y Zoología Agrícola (IMYZA)
来源
Antonie van Leeuwenhoek | 2005年 / 88卷
关键词
Isolates; PCR; RFLP;
D O I
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中图分类号
学科分类号
摘要
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identification of cry2 genes from Bacillus thuringiensis (Bt) was established. Strains from different sources of Argentina were analyzed to study the distribution of cry2 genes. The results showed that cry2Aa/cry2Ab profile was the most abundant irrespective of source and represented 56 of 59 Bt isolates (94.9%). Three different cry2 profiles were found in this collection, one of which was novel.
引用
收藏
页码:163 / 165
页数:2
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